ICC was performed to assess the expression of pluripotency markers by CA-derived primary cell lines using the PSC 4-marker ICC kit, which includes two of our iPSC markers of interest, OCT4 and SOX2, as well as two validated pluripotency markers, SSEA4 and TRA-1-60, to verify if cells expressing OCT4 and SOX2 were pluripotent. The gene discussed is POU5F1; the disease is intrahepatic cholangiocarcinoma.