NOX1 and malignant colon neoplasm: Furthermore, in the human colon cancer cell line LS513, transfection with a NOX1-specific siRNA reduced endogenous NOX1 mRNA expression by ≥75% (**p<0.01 vs. untransfected and ***p<0.001 vs. cells transfected with scrambled siRNA, Fig 1C); NOX1 protein expression was also decreased by NOX1-specific siRNA as indicated by the decreased intensity of the immunoreactive band at 65 kDa (Fig 1D).