Therefore, we next determined levels of endogenous miR-21 and ECM mediators in colon cancer cell line LS174 by RT-qPCR, and found a positive correlation between miR-21 and ECM mediators MMP-2, MMP-9, and MMP-11, but not between miR-21 and ECM inhibitor TIMP-2 (Fig. 2A). The gene discussed is TIMP2; the disease is malignant colon neoplasm.