Behavioral tests performed 30 min after CNO or NS microinjection showed that chemogenetic activation of nNOS-expressing neurons in the vmPFC caused obvious anxiety-like behaviors; this was revealed by both the OF test (Fig. 1k) and EPM test (Fig. 1l), but there was no influence on locomotor activities (Fig. 1k, l). The gene discussed is NOS1; the disease is Anxiety.