To determine the role of pPVT-vmPFC projections in hyperalgesia and anxiety-like behaviors during chronic pain, we delivered AAV-CaMKIIα-hM4Di-mCherry into the PVT 18 days before CFA injection and microinjected CNO or NS into the vmPFC 3 days after the CFA injection (Fig. 3k). This evidence concerns the gene CAMK2A and Anxiety.