Furthermore, we induced chronic neuropathic pain by SNL and found that inhibition of nNOS enzyme activity had similar anxiolytic effects and did not affect locomotor activities (Fig. 8g, h) or mechanical hyperalgesia (Fig. 8i) at day 7 after surgery, indicating the necessary role of the nNOS enzyme in different chronic pain-induced anxiety-like behaviors. Here, NOS1 is linked to Anxiety.