The analytical specificity determined using 79 serum samples from sheep/goats infected with other parasites was 98.8, 49.4, 89.9, 88.6, and 54.4% for rEg-TPx, rEg-EPC1, rEg-P29, rEg-DHFR, and HF, respectively. This evidence concerns the gene DHFR and hydrops fetalis.