The immunostaining revealed that HCLS1, EVI2B, or CD48 proteins expression were higher in pcDNA 3.3-HCLS1, pcDNA 3.3-EVI2B, or pcDNA 3.3-CD48 compared with pcDNA 3.3-NC group in xenografted tumor (Figure 13B). This evidence concerns the gene CD48 and neoplasm.