To further verify that the function of circ_100565 on NSCLC through miR-506-3p and HMGA2, we co-transfected sh-circ_100565#2 and anti-miR-506-3p or HMGA2 overexpression plasmid into A549 and H1299 cells. This evidence concerns the gene HMGA2 and non-small cell lung carcinoma.