We also analyzed HCC diagnostic performance of single biomarkers and combined panels in HBV patients who had low AFP levels (≤20 ng/μl), the TERT@Mir122 panel still sustained its diagnostic accuracy in distinguishing HCC from the other groups [CHB patients, AUC = 0.96; LC patients AUC = 0.88, patients with non-HCC, AUC = 0.94] (Fig. 4). This evidence concerns the gene TERT and laryngotracheoesophageal cleft.