To study citrulline-reactive T cell specificities with our multi-tetramer assay, we created a panel of HLA-DRB1*04:01 tetramers loaded with eight citrullinated RA-associated self peptides derived from four candidate autoantigens, namely α-enolase, vimentin, fibrinogen-β as well as CILP. Here, VIM is linked to rheumatoid arthritis.