NFE2L2 and Alzheimer disease: In an Alzheimer’s-disease model of HT-22 hippocampal cells with Aβ25–35-induced OS injury at 2 μM for 24 h, isosilybin induced the expression of Nrf2 promoted by translocation into the nucleus, thus stimulating the activity of an antioxidant response element (ARE), activating the Nrf2/ARE signaling pathway, and regulating the expression of HO-1, GST, and aldo-keto reductases 1C1 and 1C2 (AKR1C2), plus significantly inhibiting ROS production, the release of malonaldehyde (MDA) and lactate dehydrogenase (LDH), alleviating the increase of oxidative stress in this model [159].