ICMT and breast cancer: Moreover, unmethylated Ras with an alanine at the Cys-185 residue showed defective activity in inducing inflammatory responses in HEK293 and MDA-MB-231 breast cancer cells (Figure 3C,D), and interacting with MyD88 and TRIF, implying that ICMT plays a critical role in Ras function, as reported previously for Ras-dependent signaling cascades [28].