In order to understand the relationship between TIMP1 and the mesenchymal phenotype in ccRCC cells, TIMP1 and EMT represent markers (such as E-cadherin and N-) in 2 different ccRCC cell lines (A498 and Caki-1) and HK2 cells (normal renal cell lines) were examined by qRT-PCR and Western blot (Figure 6A–6D). This evidence concerns the gene TIMP1 and nonpapillary renal cell carcinoma.