The expression levels of atrial natriuretic peptide (ANP) and brain natriuretic peptide (BNP) mRNA in the left ventricle were also examined by q-PCR and were significantly increased in the TAC group compared with the control group but decreased in the LCZ696 treatment group, which indicated that LCZ696 was able to improve heart failure induced by pressure overload (Figure 1(b)). This evidence concerns the gene NPPB and heart failure.