To verify if NET1 is closely related target of miR-22, we transduced A549 and H1299 cells with miR-22 mimic or its negative control in combination with overexpressed NET1 vector in NSCLC cells without the 3′UTR and validated the expression of miR-22 and NET1 (Figures 5(a) and 5(b)). This evidence concerns the gene NET1 and non-small cell lung carcinoma.