NUAK1 and breast carcinoma: Additionally, we examined a possible connection between YAP, miR-622 and NUAK1 in breast cancer cells, given recent studies linking NUAK family to Hippo pathway.38 To this aim, MDA-MB-231 cell line was transiently transfected with YAP plasmid or with empty vector and the expression of miR-622 and NUAK1 was analysed after 48 h.