To further investigate caspase-2 in this context, various diets in combination with mouse models for NAFLD (nonalcoholic fatty liver disease) and NASH were used: high-fat diet (HFD), methionine choline-deficient diet (MCD), obese and diabetic mice, as well as MUP-uPA transgenic mice. Here, CASP2 is linked to metabolic dysfunction-associated steatotic liver disease.