The findings from the discovery profiling, AGAP2, ALPL, ANGPTL2, ATP2A1, CALML5, DNAJC5G, FDFT1, GNMT, GPT, HOXB3, KLK11, LMF1, LY6D, LYNX1, MAL, MGC16275, MRGPRF, NKPD1, SH2D3C, TNNI2, and ZNF876P, were further analysed using quantitative methylation-specific PCR (Q-MSP) in an independent large series of HNSCC specimens. The gene discussed is GPT; the disease is head and neck squamous cell carcinoma.