The findings from the discovery profiling, AGAP2, ALPL, ANGPTL2, ATP2A1, CALML5, DNAJC5G, FDFT1, GNMT, GPT, HOXB3, KLK11, LMF1, LY6D, LYNX1, MAL, MGC16275, MRGPRF, NKPD1, SH2D3C, TNNI2, and ZNF876P, were further analysed using quantitative methylation-specific PCR (Q-MSP) in an independent large series of HNSCC specimens. This evidence concerns the gene LMF1 and head and neck squamous cell carcinoma.