The findings from the discovery profiling, AGAP2, ALPL, ANGPTL2, ATP2A1, CALML5, DNAJC5G, FDFT1, GNMT, GPT, HOXB3, KLK11, LMF1, LY6D, LYNX1, MAL, MGC16275, MRGPRF, NKPD1, SH2D3C, TNNI2, and ZNF876P, were further analysed using quantitative methylation-specific PCR (Q-MSP) in an independent large series of HNSCC specimens. Here, ANGPTL2 is linked to head and neck squamous cell carcinoma.