Our current results reveal a new role for PRMT1 to promote cell survival through modulating H4R3me2a mark at the prosurvival gene ATF5. Notably, neuroblastoma cells are sensitive to PRMT1 inhibition irrespective of MYCN amplification, suggesting that additional biomarkers of response to suppression of PRMT1 activity are likely to be relevant. Here, MYCN is linked to neuroblastoma.