Although we started the application of glucose before preconditioning with Dexmedetomidine and glucose levels were already significantly increased at this time, the impact of hyperglycemia on RISK and eNOS/PKG pathways as well as ROS formation was potentially not as pronounced at this point and therefore Dexmedetomidine-induced preconditioning was still effective. Here, PRKG1 is linked to Hyperglycemia.