This result indicated that JAK-STAT pathway was activated in the Ldlr-/- mice fed with HDF, suppressed in the NASH mice treated with AAV/sh-TSLP compared with NASH mice treated with sh-TSLP control and activated in the NASH mice treated with AAV/sh-TSLP and colivelin compared with sh-TSLP group. The gene discussed is TSLP; the disease is metabolic dysfunction-associated steatohepatitis.