For the three upregulated miRNAs, the predicted hub genes were MYC, CCND1, HSP90AA1, PTEN, MAPK1, NOTCH1, CDH1, CASP3, HSPA4, and ACTB. For the five downregulated miRNAs, the predicted hub genes were AKT1, CCND1, CDC42, IL6, FN1, MAPK1, JUN, EGFR, ACTB, and HRAS. To verify potential importance in the development of ESCC, GO and KEGG analyses were also performed on these gene clusters. The gene discussed is PTEN; the disease is esophageal squamous cell carcinoma.