Consistent with those results, upregulation of miR‐140 negatively mediated the expression of histone deacetylase 4, leading to inhibited osteosarcoma cell proliferation and invasion in vitro and slowed tumor growth in vivo, which together highlighted overexpressed miR‐140 as a tumor inhibitor in osteosarcoma (Xiao et al., 2017). This evidence concerns the gene HDAC4 and osteosarcoma.