To elucidate better the effect of the miR‐140‐5p‐FBN1 axis on osteosarcoma progression, we adopted RT‐qPCR (Fig. 7A) and western blot analysis (Fig. 7B) to test the levels of FBN1 and genes linked to EMT, like E‐cadherin, N‐cadherin, and Vimentin (Fig. 7C,D). The gene discussed is CDH2; the disease is osteosarcoma.