We demonstrated that miR-22-3p overexpression reduced the expression of p-65 and TLR4, accompanied by the inhibition of HMGB1, suggesting that miR-22-3p may reduce inflammatory response and apoptosis, in sepsis-induced kidney injury rat model and LPS induced cell injury model, through HMGB1/NF-κB pathway. The gene discussed is HMGB1; the disease is Sepsis.