Phosphorylation of cyclin-dependent kinase 5 (CDK5) and peroxisome proliferator-activated receptor γ (PPARγ) was increased in adipose tissue of the ANKO mice, which led to a significant decline in gene expression of obesity-linked specific targets of phosphorylated PPARγ, including adiponectin and adipsin. This evidence concerns the gene PPARG and obesity due to melanocortin 4 receptor deficiency.