Then some mutated motifs were combined, yielding double domain mutations, including two HD/Linker mutants (Csm1dHD_LinEC and Csm1dHD_LinCC), two HD/Palm2 mutants (Csm1dHD_P2DxD and Csm1dHD_P2DD) and Csm1LinE_P2DxD, a Linker, and a Palm2 mutant (Supplementary Table S2). Here, PALM2AKAP2 is linked to Huntington disease.