We successfully established stable NPM1 silencing drug-resistant bladder cancer cell lines: the fluorescence efficiency of GFP in six kinds of cells (T24/0.8DDP Lv-NPM1, BIU-87/0.3DDP Lv-NPM1, PUMC-91/0.6DDP Lv-NPM1, and the corresponding negative controls) was observed by fluorescence microscopy, which reached more than 80%. Here, NPM1 is linked to urinary bladder carcinoma.