Repeat Foundation One testing performed on the slide from the sub-carinal lymph node revealed microsatellite stable status with a tumor mutation burden of 4 mutations/Mb, with amplifications noted in the CDK8, EPHB1, FLT3, MYC, PIK3CA, PIK3CB, RAD21 and SOX2 and loss of Rb and a MUTYH p E466 non-sense mutation. This evidence concerns the gene PIK3CA and neoplasm.