Real-time PCR showed that the transcriptional levels of Bax significantly increased and the transcriptional level of Bcl2 decreased in the rClone30-Anh(F) and rClone30-Anh(HN-F) groups compared with rClone30 and rClone30-Anh(HN) groups in the H22 tumor tissues. The gene discussed is BCL2; the disease is neoplasm.