We note that the ALS-associated mutation of TDP-43 D169G (Kabashi et al., 2008) resides within the TBD of TDP-43; however, similar to the alanine mutation in D169, the mutation to glycine had no effect on the co-immunoprecipitation of TDP-43 with Tnks-1/2 (Fig. S3E). This evidence concerns the gene TARDBP and amyotrophic lateral sclerosis.