When we analyzed the differentiation-related genes in KLF4-overexpressing non-stem cells, the expression of hepatic cancer stem cell-like genes, including CK19, EpCAM and LGR5, were significantly increased, while the expression of hepatocyte-like genes, including HNF4a, ALB and CYP3A4, were significantly decreased in these cells, suggesting that the KLF4-overexpressing cells obtained a less differentiated phenotype (Figure 2C). This evidence concerns the gene LGR5 and liver cancer.