We used the Tg(mfap4:Turquoise2)xt27 transgenic line in which macrophages are labeled turquoise and infected with wasabi-expressing M. marinum. We were able to infect the tail fins of larval zebrafish and observe recruitment of macrophages into the fin establishing a focus of infection accessible for vital staining and imaging (Fig 2B). Here, MFAP4 is linked to infection.