BACE1 and hydrops fetalis: Administration of L-NAME blunted vascular responses to ACh in RC-fed WT, DIO, and RC- and HF-fed BACE1-KO mice (Figure 2, E and F; main effect of diet, P < 0.001), demonstrating that a major proportion of the ACh-induced vascular response is NO dependent in both WT and BACE1-KO mice.