GPR50 and cancer: We did not detect any significant differences in the expression of target genes of other signaling pathways, including Wnt, Hedgehog, and Hippo, which are predominantly involved in cancer progression, stemness, and metastasis.29, 30, 31, 32, 33 Expression of other Notch pathway target genes, such as NOTCH1, MAML1, and RBPjK, was also suppressed upon GPR50 knockdown in HCC cells (Figure 3B).