To further validated the tumor suppressor role of USP38 in colorectal cancer cell, we subcutaneously injected HCT116 cells transfected with USP38 shRNA, control shRNA, control vector, and USP38 overexpression vector respectively into nude mice to evaluate the tumorigenesis of colorectal cancer cells with downregulated, normal and upregulated levels of USP38. This evidence concerns the gene USP38 and neoplasm.