Our findings that Ack1 plays a critical role in EGFR TKI resistance and that combining dasatinib with ASK120067 effectively restored the sensitivity of ASK120067 in resistant tumors provide a new set of mechanisms to support the clinical combination of EGFR TKIs with dasatinib for the treatment of EGFR-mutant NSCLC. Here, TNK2 is linked to non-small cell lung carcinoma.