Lima-Leopoldo Lima-Leopoldo AP AP Leopoldo Leopoldo AS AS Silva Silva DC DC do Nascimento do Nascimento AF AF Campos Campos DH DH Luvizotto Luvizotto RA RA et al et al Long-term obesity promotes alterations in diastolic function induced by reduction of phospholamban phosphorylation at serine-16 without affecting calcium handling Long-term obesity promotes alterations in diastolic function induced by reduction of phospholamban phosphorylation at serine-16 without affecting calcium handling J Appl Physiol (1985) J Appl Physiol (1985) 2014 2014 117 117 6 6 669 669 678 678 24970855 24970855. This evidence concerns the gene PLN and obesity due to melanocortin 4 receptor deficiency.