MACROH2A1 and infection: Control cells (CTL) were infected with a bicistronic construct expressing green fluorescent protein (GFP) and a scramble shRNA, while silencing of macroH2A1 was achieved through lentiviral infection of a bicistronic construct containing shRNA against macroH2A1 and a GFP cassette [29, 33] (knock-down, KD, Figure 1A).