To further determine the role of Septin4 in DOX-induced apoptosis of colon cancer cells, a stable knockdown of Septin4 HCT116 cell line was used to detect the expression levels of apoptosis-related proteins cleaved-PARP1 and cleaved-caspase3 by immunoblotting with and without DOX-induced apoptosis. This evidence concerns the gene PARP1 and malignant colon neoplasm.