PTEN and colorectal carcinoma: To further validate PTEN-mediated synthetic lethality to AA, a PTEN-isogenic tumor xenograft mouse model experiment was employed as shown in Fig. 6A. The growth rate of PTEN isogenic CRC cell line pair was identical in 72 h in vitro (Supplementary Fig. S4A), which precluded the affection of different growth rates of two cell lines.