However, TLR9 (Toll-like receptor 9) and NLRP3 (Nod-like receptor-family pyrin domain containing 3) may also play roles in innate immune signaling by recognizing intracellular DNA, such as mitochondrial DNA.46 To investigate whether TLR9 is required to mediate the antitumor response induced by the irradiated cell vaccine, EG7 tumor cells were implanted in the flanks of WT and Tlr9−/− mice after three immunizations with the irradiated EG7 cell vaccine. Here, TLR9 is linked to neoplasm.