Through gene set enrichment analysis (GSEA)51 of our day 12 knockdown signature, we found significant corresponding bidirectional enrichment for gene sets associated with perturbation or disruption of central mediators of the MLL leukemia program, specifically HOXA952 and glycogen synthase kinase 3, previously shown to be required for MLL leukemogenesis by Wang et al.53,54 (Fig. 7e). Here, KMT2A is linked to leukemia.