Previous studies have indicated that the majority of intermediate metabolic enzymes are acetylated and that the acetylation of metabolic enzymes plays an important role in tumorigenesis and the progression and metastasis of cancer.11,13 In our study, we determined that acetylation at K394, a site located in the active center of ENO2, played an important role in regulating ENO2 enzymatic activity and glycolytic metabolism in PDAC. This evidence concerns the gene ENO2 and cancer.