ENO2 and familial pancreatic carcinoma: Previous proteomic studies have shown that ENO2 is acetylated on multiple lysine residues.17,18 To confirm this acetylation, Flag-tagged ENO2 was ectopically expressed in HEK293T (Fig. 2a, left) and pancreatic cancer cells (Fig. 2a, right), and the acetylation level was tested using a pan-anti-acetylated lysine antibody.