Our data advocates for a scenario in which the worsening of autoimmunity in MyD88–/–Aire–/– mice could be caused by the lack of MyD88 signaling in mTECshigh, downregulation of their chemokines needed to recruit CD14+moDCs and, consequently, suboptimal production of thymic Tregs. Here, MYD88 is linked to Autoimmunity.