CASP1 and neoplasm: Thus, we chose the inflammasome components NLRP3, NLRC4, ASC, and caspase-1, which displayed increased mRNA expression levels, and then determined their protein levels in MDA-MB-231 and RT-R-MDA-MB-231 cells in response to treatment with tumor necrosis factor-α (TNF-α) or ATP, which accumulate in the tumor microenvironment.