The cytotoxicity of all the synthetic FAHFAs and authentic hydroxy fatty acids were evaluated against human hepatoma cells (C3A) by CCK-8 assay, and the values of representative species in mean ± SD (n = 6) are given as, 12-OAHSA (499 ± 2.9), 12-LAHOA (673 ± 1.7), 12-EPAHSA (415 ± 2.5), 12-EPAHOA (358 ± 2.5), EPA (117 ± 2.5), and 12-HSA (161 ± 2.7), respectively. Here, ALB is linked to hepatocellular carcinoma.