To explore the influence of altered KTN1-AS1 expression on NSCLC cells, we first performed qPCR to verify the knockdown efficiency of si-KTN1-AS1#1 and si-KTN1-AS1#2 in lung cancer cells, and the results suggested that these siRNAs had high knockdown efficiency for silencing KTN1-AS1 expression (Figure 3A). The gene discussed is KTN1; the disease is lung cancer.