Deletion of the Chr9p21 locus in the KO VSMC lines starts around exon 9 and runs downstream to the end of the coronary artery disease region.13 Analysis of ANRIL was performed by qPCR detection of ANRIL isoforms containing exons 6 and 7 (present in long and short isoforms) and exons 18 to 19 (in long isoforms only). Here, CDKN2B-AS1 is linked to coronary artery disorder.