In order to prove this phenomenon from the negative side, we further analyzed the effect of overexpressing PTPN1 on the malignant phenotype of EGFR wild‐type lung cancer cell A549, whose results showed that the inhibitory effect of PTPN1 on malignant phenotype of A549 were not remarkable compared with those in PC9 ER (Fig 4f, g, h, i and j). This evidence concerns the gene PTPN1 and lung cancer.