Consistent with this, FACS measurements of Annexin V/PI staining (Fig. 2D,E) revealed that the apoptosis rate of CD24 KD RB group cells increased by approximately 20.07 ± 1.97% in Y79 cells and 18.53 ± 0.95% in WERI‐Rb‐1 cells relative to control group cells challenged with VCR. This evidence concerns the gene CD24 and retinoblastoma.