To explore whether CD24 blunts the sensitivity of RB to VCR via autophagy pathway, we treated CD24 KD and control RB cells with CQ (an inhibitor of autophagy) or rapamycin (an inducer of autophagy acting through its inhibitory effect on mTOR) and then cultured the cells with VCR for 48 h. The gene discussed is MTOR; the disease is retinoblastoma.