To investigate whether FTSJ1 expression was associated with tRNA Am modification levels in NSCLC, FTSJ1, or si-FTSJ1 were transfected into PC9 cells, then expression levels of FTSJ1 and quantity of Am were determined by qRT-PCR and HPLC-MS assays. The gene discussed is FTSJ1; the disease is non-small cell lung carcinoma.