To evaluate the functional roles of FTSJ1 in NSCLC, overexpression of FTSJ1 was constructed with the pcDNA3.1(+) plasmid, and the expression of FTSJ1 vector was confirmed by western blot assay in PC9 and A549 cells, respectively (Fig. 4a). The gene discussed is FTSJ1; the disease is non-small cell lung carcinoma.